bio‐rad biologic duo flow chromatography system Search Results


cas  (Selleck Chemicals)
93
Selleck Chemicals cas
Cas, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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mini bio spin columns  (Bio-Rad)
94
Bio-Rad mini bio spin columns
Mini Bio Spin Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio spin 30 chromatography columns  (Bio-Rad)
95
Bio-Rad bio spin 30 chromatography columns
Bio Spin 30 Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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p 6 biospin chromatography columns  (Bio-Rad)
96
Bio-Rad p 6 biospin chromatography columns
P 6 Biospin Chromatography Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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binding mixture  (Bio-Rad)
96
Bio-Rad binding mixture
Binding Mixture, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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binding mixture - by Bioz Stars, 2026-08
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size exclusion gel permeation chromatography  (Bio-Rad)
95
Bio-Rad size exclusion gel permeation chromatography
Size Exclusion Gel Permeation Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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size exclusion gel permeation chromatography - by Bioz Stars, 2026-08
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hydroxyapatite chromatography  (Bio-Rad)
96
Bio-Rad hydroxyapatite chromatography
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Hydroxyapatite Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyapatite chromatography - by Bioz Stars, 2026-08
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bio rad p6dg gel filtration resin  (Bio-Rad)
94
Bio-Rad bio rad p6dg gel filtration resin
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Bio Rad P6dg Gel Filtration Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio gel p 6  (Bio-Rad)
94
Bio-Rad bio gel p 6
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Bio Gel P 6, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anion exchange chromatography  (Bio-Rad)
97
Bio-Rad anion exchange chromatography
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Anion Exchange Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bis tris  (Bio-Rad)
98
Bio-Rad bis tris
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Bis Tris, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ion exchange chromatography  (Bio-Rad)
95
Bio-Rad ion exchange chromatography
FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column <t>chromatography</t> steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic <t>hydroxyapatite</t> Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).
Ion Exchange Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column chromatography steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic hydroxyapatite Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).

Journal: The Journal of biological chemistry

Article Title: Lipid dependence and basic kinetics of the purified 1,2-diacylglycerol 3-glucosyltransferase from membranes of Acholeplasma laidlawii.

doi: 10.1074/jbc.272.2.929

Figure Lengend Snippet: FIG. 1. Purification of the 1,2-diacylglycerol 3-glucosyltrans- ferase in three column chromatography steps. Protein (E) and MGlcDAG synthesis activity (G) were eluted in three different column chromatography steps as described under “Materials and Methods.” A, IEC on SP-Sepharose (Pharmacia), pH 8. The solid black line is the applied NaCl concentration. B, gel filtration on Sephacryl S-100 HR (Pharmacia); one of the four best fractions from the former IEC step. C, HAC on ceramic hydroxyapatite Macro-Prep (Bio-Rad), pH 8. The solid black line is the applied KH2PO4/K2HPO4 concentration. The MGlcDAG synthesis activity was assayed as described under “Materials and Meth- ods” with 27 mM amphiphiles present (3.7 mol % DOG, 33 mol % DOPG, and 63 mol % CHAPS).

Article Snippet: Hydroxyapatite Chromatography (HAC)—A column (16 mm in diameter) packed with 6 ml of ceramic hydroxyapatite Macro-Prep (Bio-Rad) was equilibrated with HAC-buffer: 100 mM KH2PO4/K2HPO4, pH 8, 20% (v/v) glycerol, and 20 mM CHAPS.

Techniques: Purification, Column Chromatography, Activity Assay, Concentration Assay, Filtration